LOCUS       sequence-75951-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion 
            protein to induce targeted demethylation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai 
            SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson 
            MF, Joung JK
  TITLE     Targeted DNA demethylation and activation of endogenous genes using 
            programmable TALE-TET1 fusion proteins.
  JOURNAL   Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.
  PUBMED    24108092
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726."
COMMENT     Sequence Label: pJFA274
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..40
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcggccgcc accatggccc ccaagaagaa gaggaaggtg ggcattcacc gcggggtacc
       61 tatggtggac ttgaggacac tcggttattc gcaacagcaa caggagaaaa tcaagcctaa
      121 ggtcaggagc accgtcgcgc aacaccacga ggcgcttgtg gggcatggct tcactcatgc
      181 gcatattgtc gcgctttcac agcaccctgc ggcgcttggg acggtggctg tcaaatacca
      241 agatatgatt gcggccctgc ccgaagccac gcacgaggca attgtagggg tcggtaaaca
      301 gtggtcggga gcgcgagcac ttgaggcgct gctgactgtg gcgggtgagc ttagggggcc
      361 tccgctccag ctcgacaccg ggcagctgct gaagatcgcg aagagagggg gagtaacagc
      421 ggtagaggca gtgcacgcct ggcgcaatgc gctcaccggg gcccccttga acagagacga
      481 tctagagcta gacgtctcgc tgacacccga acaggtggtc gccattgcta gcaacggggg
      541 aggacggcca gccttggaga gcattgtggc ccagctgtcc aggccggacc ctgccctggc
      601 cgcgttaacc aacgaccacc tcgtcgcctt ggcctgcctc ggcggacgtc ctgcgctgga
      661 tgcagtgaaa aagggattgc cgcacgcgcc ggccttgatc aaaagaacca atcgccgtat
      721 tcccgaacgc acatcccatc gcgttgccga ccacgcgcaa gtggttcgcg tgctgggttt
      781 tttccagtgc cactcccacc cagcgcaagc atttgatgac gcc
//
