LOCUS       sequence-75958-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein 
            engineered to bind a site in the human KLF4 gene and demethylate 
            CpGs adjacent to the binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai 
            SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson 
            MF, Joung JK
  TITLE     Targeted DNA demethylation and activation of endogenous genes using 
            programmable TALE-TET1 fusion proteins.
  JOURNAL   Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.
  PUBMED    24108092
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726."
COMMENT     Sequence Label: JA412
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccgccacc atggccccca agaagaagag gaaggtgggc attcaccgcg gggtacctat
       61 ggtggacttg aggacactcg gttattcgca acagcaacag gagaaaatca agcctaaggt
      121 caggagcacc gtcgcgcaac accacgaggc gcttgtgggg catggcttca ctcatgcgca
      181 tattgtcgcg ctttcacagc accctgcggc gcttgggacg gtggctgtca aataccaaga
      241 tatgattgcg gccctgcccg aagccacgca cgaggcaatt gtaggggtcg gtaaacagtg
      301 gtcgggagcg cgagcacttg aggcgctgct gactgtggcg ggtgagctta gggggcctcc
      361 gctccagctc gacaccgggc agctgctgaa gatcgcgaag agagggggag taacagcggt
      421 agaggcagtg cacgcctggc gcaatgcgct caccggggcc cccttgaacc tgaccccaga
      481 ccaggtagtc gcaatcgcga acaataatgg gggaaagcaa gccctggaaa ccgtgcaaag
      541 gttgttgccg gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc
      601 aagcaatggg ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg
      661 tcaagcccac gggctgactc ccgatcaagt tgtagcgatt gcgaataaca atggagggaa
      721 acaagcattg gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac
      781 gcctgcacaa gtggtcgcca tcgcctcgaa tggcggcggt aagcaggcgc tggaaacagt
      841 acagcgcctg ctgcctgtac tgtgccagga tcatggactg accccagacc aggtagtcgc
      901 aatcgcgtc
//
