LOCUS       sequence-75960-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH51277
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        166..511
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     454..474
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(640..658)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatc actctgtggt ctcaggaggt caacatggtg gagcacgaca ctctggtcta
      121 ctccaaaaat gtcaaagata cagtctcaga agatcaaagg gctattgaga cttttcaaca
      181 aaggataatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
      241 aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      301 tatcattcaa gatctctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      361 catcgtggaa aaagaagagg ttccaaccac gtctacaaag caagtggatt gatgtgacat
      421 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
      481 ataaggaagt tcatttcatt tggagaggac acgctcgagt ataagagctc atttttacaa
      541 caattaccaa caacaacaaa caacaaacaa cattacaatt acatttacaa ttatcgatac
      601 aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
      661 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      781 tgagacaata accctgataa atgcttcaat aatattgaaa aa
//
