LOCUS       sequence-75970-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH78133
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(600..618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        686..790
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
      241 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt ggtctcatac
      301 tgtataagag ctcattttta caacaattac caacaacaac aaacaacaaa caacattaca
      361 attacattta caattatcga taacatggct tcttctatgc tttcttctgc tgctgttgtt
      421 gctactcgtg ctagtgctgc tcaagctagt atggttgctc cttttactgg acttaagtct
      481 gctgcttctt ttcctgttac tagaaagcaa aacaaccttg atattacttc tattgctagt
      541 aacggaggaa gagttcgagc aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
      601 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      781 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgc
//
