LOCUS       sequence-75972-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL30008
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(303..392)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             complement(405..494)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(533..551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatcactc gtggtctcac
      301 attgcgtaat ctggaacgtc atatggatac aatcctgcat agtccgggac gtcataggga
      361 tagcccgcat agtcaggaac atcgtatggg taagcagctc tagtggcgta atctggaacg
      421 tcatatggat acaatcctgc atagtccggg acgtcatagg gatagcccgc atagtcagga
      481 acatcgtatg ggtacatggt gagaccacag tggctcttca gtggacgaaa gggcctcgtg
      541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      781 gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg
      841 ca
//
