LOCUS       sequence-75982-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44300
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(612..630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        698..802
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
       61 tcattaatca ctctgtggtc tcagcttgct ctcaagatca aaggcttaaa aagctggggt
      121 tttatgaatg ggatcaaagt ttcttttttt cttttatatt tgcttctcca tttgtttgtt
      181 tcatttccct ttttgttttc gtttctatga tgcacttgtg tgtgacaaac tctctgggtt
      241 tttacttacg tctgcgtttc aaaaaaaaaa accgctttcg ttttgcgttt tagtcccatt
      301 gttttgtagc tctgagtgat cgaattgatg cctctttatt ccttttgttc cctataattt
      361 ctttcaaaac tcagaagaaa aaccttgaaa ctctttgcaa tgttaatata agtattgtat
      421 aagattttta ttgatttggt tattagtctt acttttgcta cctccatctt cacttggaac
      481 tgatattctg aatagttaaa gcgttacatg tgttccattc acaaatgaac ttaaactagc
      541 acaaagtcag atattttaag atcgcaccat ttcgcttgag accacgaagt ggctcttcag
      601 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      781 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      841 cagcggtggt aacggcgcag tg
//
