LOCUS       sequence-75996-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) 
            fusion protein engineered to bind a site in the human beta-globin 
            (HBB) gene for use as a control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai 
            SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson 
            MF, Joung JK
  TITLE     Targeted DNA demethylation and activation of endogenous genes using 
            programmable TALE-TET1 fusion proteins.
  JOURNAL   Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.
  PUBMED    24108092
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726."
COMMENT     Sequence Label: MLM3739
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..82
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             89..109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcggccgcc accatggact acaaagacca tgacggtgat tataaagatc atgacatcga
       61 ttacaaggat gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcaccg
      121 cggggtacct atggtggact tgaggacact cggttattcg caacagcaac aggagaaaat
      181 caagcctaag gtcaggagca ccgtcgcgca acaccacgag gcgcttgtgg ggcatggctt
      241 cactcatgcg catattgtcg cgctttcaca gcaccctgcg gcgcttggga cggtggctgt
      301 caaataccaa gatatgattg cggccctgcc cgaagccacg cacgaggcaa ttgtaggggt
      361 cggtaaacag tggtcgggag cgcgagcact tgaggcgctg ctgactgtgg cgggtgagct
      421 tagggggcct ccgctccagc tcgacaccgg gcagctgctg aagatcgcga agagaggggg
      481 agtaacagcg gtagaggcag tgcacgcctg gcgcaatgcg ctcaccgggg cccccttgaa
      541 cctgacccca gaccaggtag tcgcaatcgc gtcacatgac gggggaaagc aagccctgga
      601 aaccgtgcaa aggttgttgc cggtcctttg tcaagaccac ggccttacac cggagcaagt
      661 cgtggccatt gcaagcaatg ggggtggcaa acaggctctt gagacggttc agagacttct
      721 cccagttctc tgtcaagccc acgggctgac tcccgatcaa gttgtagcga ttgcgtccaa
      781 cggtggaggg aaacaagcat tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc
      841 ccacggtttg acgcctgcac aagtggtcgc catcgccagc catgatggcg gtaagcaggc
      901 gctggaaaca gtacagcgcc tgctgcctgt actgtgcc
//
