LOCUS       sequence-76000-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45180
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(160..264)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     332..350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
       61 ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      121 gcgttcggtc aaggttctgg accagttgcg tgagcgcata ctcttccttt ttcaatatta
      181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
      301 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtcc
      361 actgaagagc cacttcgtgg tctcaagtag ccctcatgat caaagggtcc atgattcatg
      421 ataacgtcgt ttactgccac aggattaagg aaaggtgttg acacccttat cggtacgcac
      481 cactaggata aggccacgtg gcagaaaact ccattatctt atcttttctc ttgtggggga
      541 aatgaagtga atgtgcggtt actggtctgc acttgatcgg ttgcttttgg ctcctttcgt
      601 ttgggagaaa agaaaccaga cgcattacct taaatttaat gcttttggtc ttttggacct
      661 cttatttacc atgtatgatt taatttggct taacactttc tttggaaata aggtcgcatt
      721 aaaaaatagt tataacaaaa ttatttaaat ttgatattta cataaatata ttgtatgata
      781 tttatccgga caaaaaaaa
//
