LOCUS       sequence-76001-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) 
            fusion protein engineered to bind a site in the human beta-globin 
            (HBB) gene for use as a control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai 
            SQ, Ho QH, Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson 
            MF, Joung JK
  TITLE     Targeted DNA demethylation and activation of endogenous genes using 
            programmable TALE-TET1 fusion proteins.
  JOURNAL   Nat Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726.
  PUBMED    24108092
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Oct 9. doi: 10.1038/nbt.2726."
COMMENT     Sequence Label: MLM3743
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14..79
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             86..106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccgccacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
       61 caaggatgac gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg
      121 ggtacctatg gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa
      181 gcctaaggtc aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac
      241 tcatgcgcat attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa
      301 ataccaagat atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg
      361 taaacagtgg tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag
      421 ggggcctccg ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt
      481 aacagcggta gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct
      541 gaccccagac caggtagtcg caatcgcgtc gaacattggg ggaaagcaag ccctggaaac
      601 cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt
      661 ggccattgca agcaatgggg gtggcaaaca ggctcttgag acggttcaga gacttctccc
      721 agttctctgt caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgaataacaa
      781 tggagggaaa caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca
      841 cggtttgacg cctgcacaag tggtcgccat cgccagccat gatggcggta agcaggcgct
      901 ggaaacagta cagcgcctgc tgcctgtact gtgcc
//
