LOCUS       sequence-76013-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50004
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(159..263)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     331..349
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
       61 cccaaaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
      121 cgttcggtca aggttctgga ccagttgcgt gagcgcatac tcttcctttt tcaatattat
      181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
      301 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtcca
      361 ctgaagagcc acgtggtctc aaagctcact tgtacagctc gtccatgccg ccggtggagt
      421 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
      481 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
      541 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
      601 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
      661 tggttttctt ctgcattacg gggccgtcgg a
//
