LOCUS       sequence-76021-e        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-Rab1AS25N (dominant negative mutant) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Huang B, Hubber A, McDonough JA, Roy CR, Scidmore MA, Carlyon JA
  TITLE     The Anaplasma phagocytophilum-occupied vacuole selectively recruits 
            Rab-GTPases that are predominantly associated with recycling 
            endosomes.
  JOURNAL   Cell Microbiol. 2010 Sep 1;12(9):1292-307. doi: 
            10.1111/j.1462-5822.2010.01468.x. Epub 2010 Mar 25.
  PUBMED    20345488
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2010.01468.x"; journalName: "Cell Microbiol"; 
            date: "2010-09-1- 1"; volume: "12"; issue: "9"; pages: "1292-307"
COMMENT     Sequence Label: EGFP-Rab1AS25N
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    813..934
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(850..869)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     904..923
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1078..1099)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc caccatgtcc agcatgaatc
       61 ccgaatatga ttatttattc aagttacttc tgattggcga ctcaggggtt ggaaagaatt
      121 gccttcttct taggtttgca gatgatacat atacagaaag ctacatcagc acaattggtg
      181 tggatttcaa aataagaact atagagttag acgggaaaac aatcaagctt caaatatggg
      241 acacagcagg ccaggaaaga tttcgaacaa tcacctccag ttattacaga ggagcccatg
      301 gcatcatagt tgtgtatgat gtgacagatc aggagtcctt caataatgtt aaacagtggc
      361 tgcaggaaat agatcgttat gccagtgaaa atgtcaacaa attgttggta gggaacaaat
      421 gtgatctgac cacaaagaaa gtagtagact acacaacagc gaaggaattt gctgattccc
      481 ttggaattcc gtttttggaa accagtgcta agaatgcaac gaatgtagaa cagtctttca
      541 tgacgatggc agctgagatt aaaaagcgaa tgggtcccgg agcaacagct ggtggtgctg
      601 agaagtccaa tgttaaaatt cagagcactc cagtcaagca gtcaggtgga ggttgctgct
      661 aactcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
      721 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      781 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      841 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      901 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
      961 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1021 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1081 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
//
