LOCUS       sequence-76041-e        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-Rab10T23N (dominant negative mutant) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Huang B, Hubber A, McDonough JA, Roy CR, Scidmore MA, Carlyon JA
  TITLE     The Anaplasma phagocytophilum-occupied vacuole selectively recruits 
            Rab-GTPases that are predominantly associated with recycling 
            endosomes.
  JOURNAL   Cell Microbiol. 2010 Sep 1;12(9):1292-307. doi: 
            10.1111/j.1462-5822.2010.01468.x. Epub 2010 Mar 25.
  PUBMED    20345488
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2010.01468.x"; journalName: "Cell Microbiol"; 
            date: "2010-09-1- 1"; volume: "12"; issue: "9"; pages: "1292-307"
COMMENT     Sequence Label: EGFP-Rab10T23N
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    798..919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(835..854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     889..908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1063..1084)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gactcagatc caccatggcg aagaagacgt
       61 acgacctgct tttcaagctg ctcctgatcg gggattccgg agtggggaag aactgcgtcc
      121 tttttcgttt ttcggatgat gccttcaata ctacctttat ttccaccata ggaatagact
      181 tcaagatcaa aacagttgaa ttacaaggaa agaagatcaa gctacagata tgggatacag
      241 caggccagga gcgatttcac accatcacaa cctcctacta cagaggcgca atgggtatca
      301 tgctagtata tgacatcacc aatggtaaaa gttttgaaaa catcagcaaa tggcttagaa
      361 acatagatga gcatgccaat gaagatgtgg aaagaatgtt actaggaaac aagtgtgata
      421 tggacgacaa aagagttgta cctaaaggaa aaggagaaca gattgcaagg gagcatggta
      481 ttaggttttt tgagactagt gcaaaagcaa atataaacat cgaaaaggcg ttcctcacgt
      541 tagctgaaga tatccttcga aagacccctg taaaagagcc caacagtgaa aatgtagata
      601 tcagcagtgg aggaggcgtg acaggctgga agagcaaatg ctgctgactc gacggtaccg
      661 cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
      721 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      781 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      901 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
      961 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1021 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1081 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
//
