LOCUS       sequence-76044-e        2109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1 wild type with FLAG tag 
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1WT-CMyc
FEATURES             Location/Qualifiers
     source          1..2109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..76
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2042..2071
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cwwgagacac cgggaccgat ccagcctccg cggccccgaa ctagtcacca tggactacaa
       61 ggacgacgat gacaaaatgg cgtccacgga ttacagtacc tatagccaag ctgcagcgca
      121 gcagggctac agtgcttaca ccgcccagcc cactcaagga tatgcacaga ccacccaggc
      181 atatgggcaa caaagctatg gaacctatgg acagcccact gatgtcagct atacccaggc
      241 tcagaccact gcaacctatg ggcagaccgc ctatgcaact tcttatggac agcctcccac
      301 tggttatact actccaactg ccccccaggc atacagccag cctgtccagg ggtatggcac
      361 tggtgcttat gataccacca ctgctacagt caccaccacc caggcctcct atgcagctca
      421 gtctgcatat ggcactcagc ctgcttatcc agcctatggg cagcagccag cagccactgc
      481 acctacaaga ccgcaggatg gaaacaagcc cactgagact agtcaacctc aatctagcac
      541 agggggttac aaccagccca gcctaggata tggacagagt aactacagtt atccccaggt
      601 acctgggagc taccccatgc agccagtcac tgcacctcca tcctaccctc ctaccagcta
      661 ttcctctaca cagccgacta gttatgatca gagcagttac tctcagcaga acacctatgg
      721 gcaaccgagc agctatggac agcagagtag ctatggtcaa caaagcagct atgggcagca
      781 gcctcccact agttacccac cccaaactgg atcctacagc caagctccaa gtcaatatag
      841 ccaacagagc agcagctacg ggcagcagag ttcattccga caggaccacc ccagtagcat
      901 gggtgtttat gggcaggagt ctggaggatt ttccggacca ggagagaacc ggagcatgag
      961 tggccctgat aaccggggca ggggaagagg gggatttgat cgtggaggca tgagcagagg
     1021 tgggcgggga ggaggacgcg gtggaatggg cgctggagag cgaggtggct tcaataagcc
     1081 tggtggaccc atggatgaag gaccagatct tgatctaggc ccacctgtag atccagatga
     1141 agactctgac aacagtgcaa tttatgtaca aggattaaat gacagtgtga ctctagatga
     1201 tctggcagac ttctttaagc agtgtggggt tgttaagatg aacaagagaa ctgggcaacc
     1261 catgatccac atctacctgg acaaggaaac aggaaagccc aaaggcgatg ccacagtgtc
     1321 ctatgaagac ccacccactg ccaaggctgc cgtggaatgg tttgatggga aagattttca
     1381 agggagcaaa cttaaagtct cccttgctcg gaagaagcct ccaatgaaca gtatgcgggg
     1441 tggtctgcca ccccgtgagg gcagaggcat gccaccacca ctccgtggag gtccaggagg
     1501 cccaggaggt cctgggggac ccatgggtcg catgggaggc cgtggaggag atagaggagg
     1561 cttccctcca agaggacccc ggggttcccg agggaacccc tctggaggag gaaacgtcca
     1621 gcaccgagct ggagactggc agtgtcccaa tccgggttgt ggaaaccaga acttcgcctg
     1681 gagaacagag tgcaaccagt gtaaggcccc aaagcctgaa ggcttcctcc cgccaccctt
     1741 tccgcccccg ggtggtgatc gtggcagagg tggccctggt ggcatgcggg gaggaagagg
     1801 tggcctcatg gatcgtggtg gtcccggtgg aatgttcaga ggtggccgtg gtggagacag
     1861 aggtggcttc cgtggtggcc ggggcatgga ccgaggtggc tttggtggag gaagacgagg
     1921 tggccctggg gggccccctg gacctttgat ggaacagatg ggaggaagaa gaggaggacg
     1981 tggaggacct ggaaaaatgg ataaaggcga gcaccgtcag gagcgcagag atcggcccta
     2041 cgaacaaaaa ctcatctcag aagaggatct gtasgcggcc gcactcgaga tatctagacc
     2101 cagccttta
//
