LOCUS       sequence-76054-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Flag tagged mouse Tim3 (T2 truncation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Lee J, Su EW, Zhu C, Hainline S, Phuah J, Moroco JA, Smithgall TE, 
            Kuchroo VK, Kane LP
  TITLE     Phosphotyrosine-dependent coupling of Tim-3 to T-cell receptor 
            signaling pathways.
  JOURNAL   Mol Cell Biol. 2011 Oct;31(19):3963-74. doi: 10.1128/MCB.05297-11. 
            Epub 2011 Aug 1.
  PUBMED    21807895
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.05297-11"; journalName: "Mol Cell Biol"; date: 
            "2011-10"; volume: "31"; issue: "19"; pages: "3963-74"
COMMENT     Sequence Label: Flag‐mTim3 (T2 truncation)
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attagcttgg taccgagctc ggatccatgg ccttaccagt gaccgccttg ctcctgccgc
       61 tagccttgct gctccacgcc gccaggccgg attacaagga cgatgacgac aagatcgatt
      121 tggaaaatgc ttatgtgttt gaggttggta agaatgccta tctgccctgc agttacactc
      181 tatctacacc tggggcactt gtgcctatgt gctggggcaa gggattctgt ccttggtcac
      241 agtgtaccaa cgagttgctc agaactgatg aaagaaatgt gacatatcag aaatccagca
      301 gataccagct aaagggcgat ctcaacaaag gagacgtgtc tctgatcata aagaatgtga
      361 ctctggatga ccatgggacc tactgctgca ggatacagtt ccctggtctt atgaatgata
      421 aaaaattaga actgaaatta gacatcaaag cagccaaggt cactccagct cagactgccc
      481 atggggactc tactacagct tctccaagaa ccctaaccac ggagagaaat ggttcagaga
      541 cacagacact ggtgaccctc cataataaca atggaacaaa aatttccaca tgggctgatg
      601 aaattaagga ctctggagaa acgatcagaa ctgctatcca cattggagtg ggagtctctg
      661 ctgggttgac cctggcactt atcattggtg tcttaatcct taaatggtat tcctgtaaga
      721 aaaagaagtt atcgagtttg agccttatta cactggccaa cttgcctcca ggagggttgg
      781 caaatgcagg agcagtcagg attcgctctg aggaaaatat ctgacagaat tccagctgag
      841 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
      901 atgtctgcc
//
