LOCUS       sequence-76069-e        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-Rab33A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Huang B, Hubber A, McDonough JA, Roy CR, Scidmore MA, Carlyon JA
  TITLE     The Anaplasma phagocytophilum-occupied vacuole selectively recruits 
            Rab-GTPases that are predominantly associated with recycling 
            endosomes.
  JOURNAL   Cell Microbiol. 2010 Sep 1;12(9):1292-307. doi: 
            10.1111/j.1462-5822.2010.01468.x. Epub 2010 Mar 25.
  PUBMED    20345488
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1462-5822.2010.01468.x"; journalName: "Cell Microbiol"; 
            date: "2010-09-1- 1"; volume: "12"; issue: "9"; pages: "1292-307"
COMMENT     Sequence Label: EGFP-Rab33A
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(735..746)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    933..1054
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(970..989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1024..1043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtccg gccggactca gatctcgagc tcaagcttcg
       61 aattcaccat ggcgcagccc atcctgggcc atgggagcct gcagcccgcc tcggccgctg
      121 gcctggcgtc cctggagctc gactcgtcgc tggaccagta cgtgcagatt cgcatcttca
      181 aaataatcgt gattggggac tccaacgtgg gcaagacctg cctgaccttc cgcttctgcg
      241 ggggtacctt cccagacaag actgaagcca ccatcggcgt ggacttcagg gagaagaccg
      301 tggaaatcga gggcgagaag atcaaggttc aggtgtggga cacagcaggt caggaacgtt
      361 tccgcaaaag catggtcgag cattactacc gcaacgtaca tgccgtggtc ttcgtctatg
      421 acgtcaccaa gatgacatct ttcaccaacc tcaaaatgtg gatccaagaa tgcaatgggc
      481 atgctgtgcc cccactagtc cccaaagtgc ttgtgggcaa caagtgtgac ttgagggaac
      541 agatccaggt gccctccaac ttagccctga aatttgctga tgcccacaac atgctcttgt
      601 ttgagacatc ggccaaggac cccaaagaga gccagaacgt ggagtcgatt ttcatgtgct
      661 tggcttgccg attgaaggcc cagaaatccc tgctgtatcg tgatgctgag aggcagcagg
      721 ggaaggtgca gaaactggag ttcccacagg aagctaacag taaaacttcc tgtccttgtt
      781 gactcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
      841 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      901 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      961 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1021 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     1081 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1141 atcggcaaaa tcccttataa atcaaaagaa
//
