LOCUS       sequence-76077-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged ER D538G mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Toy W, Shen Y, Won H, Green B, Sakr RA, Will M, Li Z, Gala K, 
            Fanning S, King TA, Hudis C, Chen D, Taran T, Hortobagyi G, Greene 
            G, Berger M, Baselga J, Chandarlapaty S
  TITLE     ESR1 ligand-binding domain mutations in hormone-resistant breast 
            cancer.
  JOURNAL   Nat Genet. 2013 Dec;45(12):1439-45. doi: 10.1038/ng.2822. Epub 2013 
            Nov 3.
  PUBMED    24185512
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2822"; 
            journalName: "Nat Genet"; date: "2013-12"; volume: "45"; issue: 
            "12"; pages: "1439-45"
COMMENT     Sequence Label: pcDNA-HA-ER D538G
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             131..157
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cgccaccatg tatccatatg acgtcccaga ctatgccacc atgaccctcc acaccaaagc
      181 atctgggatg gccctactgc atcagatcca agggaacgag ctggagcccc tgaaccgtcc
      241 gcagctcaag atccccctgg agcggcccct gggcgaggtg tacctggaca gcagcaagcc
      301 cgccgtgtac aactaccccg agggcgccgc ctacgagttc aacgccgcgg ccgccgccaa
      361 cgcgcaggtc tacggtcaga ccggcctccc ctacggcccc gggtctgagg ctgcggcgtt
      421 cggctccaac ggcctggggg gtttcccccc actcaacagc gtgtctccga gcccgctgat
      481 gctactgcac ccgccgccgc agctgtcgcc tttcctgcag ccccacggcc agcaggtgcc
      541 ctactacctg gagaacgagc ccagcggcta cacggtgcgc gaggccggcc cgccggcatt
      601 ctacaggcca aattcagata atcgacgcca gggtggcaga gaaagattgg ccagtaccaa
      661 tgacaaggga agtatggcta tggaatctgc caaggagact cgctactgtg cagtgtgcaa
      721 tgactatgct tcaggctacc attatggagt ctggtcctgt gagggctgca aggccttctt
      781 caagagaagt attcaaggac ataacgacta tatgtgtcca gccaccaacc agtgcaccat
      841 tgataaaaac aggaggaaga gctgccaggc ctgccggctc cgcaaatgct acgaagtggg
      901 aatgatgaaa ggtgggatac gaaaagaccg aagaggaggg agaatgttga aacacaagcg
      961 ccaga
//
