LOCUS       sequence-76078-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot 
            JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
            Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen 
            HS, Mercola M
  TITLE     Lentiviral vectors and protocols for creation of stable hESC lines 
            for fluorescent tracking and drug resistance selection of 
            cardiomyocytes.
  JOURNAL   PLoS ONE. 2009  . 4(4):e5046.
  PUBMED    19352491
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2009  . 4(4):e5046."
COMMENT     Sequence Label: PGK-H2BmCherry
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..489)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSAK"
     primer_bind     complement(567..585)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
       61 ctccttgatg atggccatgt tatcctcctc gcccttgctc accatcggat ccttagcgct
      121 ggtgtacttg gtgacggcct tagtaccctc ggacacggcg tgcttggcca actccccagg
      181 cagcagcagg cgcacggccg tctggatctc cctggaggtg atggtcgagc gcttgttgta
      241 atgcgccagg cgggaagcct cacctgcgat gcgctcgaaa atgtcgttca caaacgaatt
      301 catgatgccc atggccttgg acgaaatgcc ggtgtcaggg tggacctgct tcagaacctt
      361 gtacacatag atggaatagc tctccttgcg gctgcgcttg cgcttcttgc cgtctttctt
      421 ctgcgcctta gtcaccgcct tcttggagcc ctttttcggg gcgggagcag acttcgctgg
      481 ctctggcatg gtggcggatc cccctgggga gagaggtcgg tgattcggtc aacgagggag
      541 ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac
      601 agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc
      661 tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc
      721 attgctccct ggcgctgtcc gtctgcga
//
