LOCUS       sequence-76080-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat3
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..193
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             209..226
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata tacatatgaa agaaaccgct gctgctaaat tcgaacgcca
      181 gcacatggac agcccagatc tgggtaccct ggtgccacgc ggttccatgg cgatatcgga
      241 tcgggaaggt gtgaatgaca atgaagaagg tttcttttcc gcccgcgcaa tcgtgggtgg
      301 catcgctcgc ctgatcgact caattatccc ggaaccgctg ggcggtgcgc atcgcattaa
      361 cgccgaagat ccgaatacct ttctgccgtc tccgggcaaa acgaacgtgg acctgcagat
      421 tcgcgcactg atcggcttcg ctggtccgcg tctggcatcc atcctggcaa aggattcatg
      481 gtatgatctg gacgctcact acgacgcgct ggaaacccgt ttttggcagt tctatccgcg
      541 cacgcatcac caaccggtgc gtctgtgggg cgaagccctg aaaattacca cggttcaagc
      601 ggccattgat tatatcaacg gtcatcaggc atggatctac tccgtgtcaa aagtggttga
      661 aagctctagt tattacggca agggtaccgc gatttttagc ttccacgcaa tcaaagctcc
      721 gcaggcagaa gttctgacgc cgggttataa ggccgatgaa ggctacgacg tcgtgggtac
      781 cgtccgtatt tatacggatg tgcgctccaa tgcagcttca ctgtacggtt gggacatcct
      841 gctggcaggt accgcatggc cgggtaaagc cgaagatttt aacggttggc tgctgaatcg
      901 tctgtggcat gaaccggtct cgccgcgtaa caatccgctg gcactgcgtt ttg
//
