LOCUS       sequence-76081-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat4
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    66..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             138..182
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             198..215
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact
       61 ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca
      181 gcccagatct gggtaccctg gtgccacgcg gttccatggc gatatcggat cgggaaggcg
      241 tgaatgacaa tgaagaaggc tttttctctg ctcgtgacgt tatcgccgaa ccgtatcgtg
      301 tggctgactg gctgggtaaa ggcgaactgg tggttctggg tcgtttcttt gcggccctgg
      361 cccgcaacaa tgcaggcgaa accgtgctgc tgggtcgtat tctggatgaa gcgggcctgc
      421 cgggtgaact gcgtcagttt gacgtcgtgc gcttcctggt ggcccagagt ggcgttctga
      481 ttacccaagt ccgtctggat tttgtgacgc gcgttgtcat cctgtatccg cgcctggtcc
      541 tggtggaatc accgtcgaat ccgctgctgc gtgcagctgc gggcattagc gaaaccctgc
      601 tgcgcgaagg tagctctctg ctgggctctg acgcaggtga actggcaggt gccggtaaag
      661 tgccgggtac catcggtttt gctacggtcc gtctgggcgt gaccacgagc tggttcgatc
      721 cgctgattgg tgcggacatc gttaaggtcg ctgaatggct ggcagaacat ccgcaggtgg
      781 cacgtctgct gagcaactct ccgctgcagg ttgatattca actgctgcgt ggctttgatg
      841 acagctt
//
