LOCUS       sequence-76097-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat11
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             139..183
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             199..216
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
      181 agcccagatc tgggtaccct ggtgccacgc ggttccatgg cgatatcgga tcgggaaggc
      241 gtgaatgaca atgaagaagg ctttttctcc gctcgtggct ataccgtctc gatttttaac
      301 cgtgctgctg tgctgccggc caacctgatt caggctcaac gtaccctgtg ggtgccggcg
      361 ctgaaatatc tgatcgcagc cggtcagaag ctgggtgcag ctgttgccac gctgaaaagc
      421 ggctttattt tcatcggtcc gaagaacgct ctgcaggaac tgattatcga tggtattaaa
      481 gtcattgcaa tcgatgtgaa tgacgaacaa ctgaagctgg ttctggatgg catcgaagtg
      541 gttggctctc tggtcggtac ccgcaatggc ggtgttgcgg gtaacaccac ggtcaatcag
      601 aaaagcggct ctggtaccct gaccgtcagt aacaccacgc tgacgcaaaa gattgaatat
      661 gtgtaccagt ccgcagaaca actgcgtaat gcagatgcac tgaccctgca ggctccggca
      721 caacgtgcaa cgtttgtcgt ggacccgcag ggtattatcc aagccattga agtgaccgca
      781 gaaggcatcg gtcgtgaagg cgaagctacg ctggcaccga gcctggatct ggtgggtaaa
      841 tctattatcg ttgccaccgg tgccaaagca agtctgtccg catttgatta tctgattcgc
      901 atc
//
