LOCUS       sequence-76099-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat17
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             147..191
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             207..224
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata catatgaaag aaaccgctgc tgctaaattc gaacgccagc
      181 acatggacag cccagatctg ggtaccctgg tgccacgcgg ttccatggcg atatcggatc
      241 gggaaggtgt gaatgataat gaagaaggct ttttctcagc acgcggtagc aatgattttg
      301 tgaccaatgt ggataaacag tatgcgacga cctatattaa catcgttggc aaaacgggtg
      361 atatcggcct gtttcgcctg ctggttagcg aactgtccgg tgtcgaaccg aagttcctga
      421 atgcggaaat tatcgaagat attctggacc gttttggctt cctgctggat gcgctgaaaa
      481 ccgccctgta cagttggctg tttgcgcgct tcgccaaccc gcaggaaggt tccgtggttt
      541 ttgatgacca aattcgtgtc gcagtggcaa ccatcggtgc agtgctgccg ggtgatttca
      601 aaggcgacct ggaaagtgtc ctggatctga ccggcaaagt gattccggcg acgattctgg
      661 gcatccagtc agatcgctcg gacgaagtgc tgtccgaccg ttttaccgcg gccgaattcc
      721 gcggtgttca gtggattccg gattggggcc aagcacgtgc ttcattcgtt acgctgcagg
      781 acgtgggcgg tcgtgttttt gaaattaacc gcgcctcgtt cgtgaccctg caagatgtcg
      841 gcggtcgtgt gtttgaaatc aaccgcaatt atacgattgg tgatgtcatc gcgcgcaact
      901 gggtttctcc ggtcgacgcc atcgtggaac gtattgatga catcgatctg aatctgg
//
