LOCUS       sequence-76103-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-tagged ER Y537S mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Toy W, Shen Y, Won H, Green B, Sakr RA, Will M, Li Z, Gala K, 
            Fanning S, King TA, Hudis C, Chen D, Taran T, Hortobagyi G, Greene 
            G, Berger M, Baselga J, Chandarlapaty S
  TITLE     ESR1 ligand-binding domain mutations in hormone-resistant breast 
            cancer.
  JOURNAL   Nat Genet. 2013 Dec;45(12):1439-45. doi: 10.1038/ng.2822. Epub 2013 
            Nov 3.
  PUBMED    24185512
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2822"; 
            journalName: "Nat Genet"; date: "2013-12"; volume: "45"; issue: 
            "12"; pages: "1439-45"
COMMENT     Sequence Label: pcDNA-HA-ER Y537S
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..890)
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLSDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGSLPR"
ORIGIN
        1 gaccgtggca gggaaccctc tgcctccccc gtgatgtaat acttttgcaa ggaatgcgat
       61 gaagtagagc ccgcagtggc caagtggctt tggtccgtct cctccacgga tgcccctcca
      121 cggctagtgg gcgcatgtag gcggtgggcg tccagcatct ccagcagcag gtcactgagg
      181 ggcaccacgt tcttgcactt catgctgtac agatgctcca tgcctttgtt actcatgtgc
      241 ctgatgtggg agaggatgag gaggagctgg gccagccgct ggtgctgctg ctgcagggtc
      301 aggcctgcct tggccatcag gtggatcaaa gtgtctgtga tcttgtccag gactcggtgg
      361 atatggtcct tctcttccag agacttcagg gtgctggaca gaaatgtgta cactccagaa
      421 ttaagcaaaa taatagattt gaggcacaca aactcctctc cctgcagatt catcatgcgg
      481 aaccgagatg atgtagccag cagcatgtcg aagatctcca ccatgccctc tacacatttt
      541 ccctggttcc tgtccaagag caagttagga gcaaacagta gcttccctgg gtgctccatg
      601 gagcgccaga cgagaccaat catcaggatc tctagccagg cacattctag aaggtggacc
      661 tgatcatgga gggtcaaatc cacaaagcct ggcaccctct tcgcccagtt gatcatgtga
      721 accagctccc tgtctgccag gttggtcagt aagcccatca tcgaagcttc actgaagggt
      781 ctggtaggat catactcgga atagagtatg gggggctcag catccaacaa ggcactgacc
      841 atctggtcgg ccgtcaggga caaggccagg ctgttcttct tagagcgttt gatcatgagc
      901 gggcttggcc aaaggttggc agctctcatg tc
//
