LOCUS       sequence-76106-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat14
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agacgtgccg
       61 gcagaccaga tgccgtatta ccgctcagtt tccagaacgc gatttcgccc agttcttcca
      121 gaatggtacg agaaccgatg ccaatagcac caaagcgatc tgccaggcct gcggcaacga
      181 tattgcgacc ggtcgggtta ataaagaaac gttcttcgcc cagttcggat gcgatgacac
      241 gcagattgat ttgaccatca atgacgcggt tctggtaact aatgaaaaca cctttaaagc
      301 cttgatactg aatccaggcc ttaatcaggt caaccaggaa cgtcaggcct gctttatcca
      361 gggccgggat ttcttcccag gtcttgtcaa tttcatccag cagaaacagc gggttgcgaa
      421 ccagcacttc ttgttcacgc agttcgtaga tattgttcac cgccggcagt ttcgacggtg
      481 agtccacata aatgacttcc ttcagggctt cgatcgcagc ctggctatca atcagcgcac
      541 ctttgcccgt accaatcaca cccgcggtct tgacagcttg gccctgggac agttgcagca
      601 gacgatcaac actggtctga cccagcaggc ggtcgacgcc aacagagctc aggaaatccg
      661 ccacgacacg acccactgct gcgtcaccca gagaggtata cagtttagcc ggcgtaccgg
      721 tatcgatgct tgcaaaaacc acttccttgc ccacttcacc gcccacagag accgtgcgat
      781 ccagaatttc tgcggattta ctggcggtgt agctcggaac cttcacattg ataacggcac
      841 ccgttttgaa cacatacgga atgcccaggc
//
