LOCUS       sequence-76108-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat16
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agcttggcac
       61 ccgggccaga gaattcgata cccagctgcg gcagacggct ttcccacagg gtcagttctt
      121 tcacttgcgc aatcacatcg tcgacgctac gggccgcagc ttcatcccaa ttcacgacgt
      181 tccagaattc cttgaagtta tcaaccgaac cgaagtcgcg atagttcacc gggtccagaa
      241 agcctgattg cttctgaatg tccggttgat tgttaccctt ctgggcgccc gcgtagataa
      301 caccatactt atcgcccaga atattcagcg gcactgctgc gaactggcca cgcagaacca
      361 cttcgtaaac atcgtcagcc agttgggaac ttgcggtatc caggtccagt ttgacttccg
      421 gctgccaatc gcggtcaaaa acgttaatac gggaatcttc gacggtaaca ctggccagtt
      481 ggcgcacgtc caggatctga ttgttaatca gaccttcttc gatttcacgc agagcaatga
      541 ccgtggtttt gccttcgatc agaaccggtt caaatgcctg aatacccagg cgcagatacg
      601 ggccaaccgt gatcaccgaa cgaatttggc ctgaggtcgg cagatcaccc agacgaccca
      661 ctgcggacag gtcatatttc agcagttcca cttccgtgcg ggtactcgcc gggacgtaac
      721 cacggttgat atccgtaatt tccagttcgc cacgcggcgt atcctgcggg gtagagataa
      781 tcaggatgtc gcgaatgacg gtacccagag caataactgc atcataacgc gcag
//
