LOCUS       sequence-76109-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat17
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gcttcagcgg
       61 gccatcagca attgcgacac cgtaagcttg caggaattca gcattttttg caaccagcgt
      121 aaaggtctgt gcacgataca gttcttcaac cagcggagcg cgcagcacga agtatgcgtc
      181 agaacggccg ctttcaaagg tcacatacgc caggcgatcc agcggattga aacggcccag
      241 cagaaaagaa ccaatttcca cagcgcgttc cagaccttcc agatcaaagt tgcccttaac
      301 cagaccttcg attgcttggc ttttgtattc acccggcaca taggtcggtg cacctgccgg
      361 attgatcttg aacagcagca gcgtcggaat gcctttcggg gcggtacccg gattctggtc
      421 gatgttcagt ttttcatcca cgccttccgg cagaaacgca atgtccttcg gatcttccag
      481 cagaccgata acattgtttt tcggaatgaa cggttccggc acgcgggcca gcgtctgcag
      541 aaagccacgg ttttccactt ccagatcgct gatcgcggtt ttaatcggca ggtcatcttc
      601 gtcgataatt tcgccgcgca gcggttcgat atcttcacca cgttcaaccg tttcaatgcc
      661 cagggtatcc agacccagct tcacggcacc aatgccgaca gcatttgcca gtttttcaaa
      721 cagcgccagg ttttcttcca cactacggat ttgttccggc gtcagttcgt cgacgtatgc
      781 caggccttta accagatatt ccacaatatc accggtgcgg accagcagag caacctgcgg
      841 aaaagagctg ccttcgactg cacggaacgt cggaccaaag gtatagatgc gggcaaccgc
      901 gccaatca
//
