LOCUS       sequence-76110-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agcttcaggg
       61 ttgccagcag atcattaaat ttcagggcac cgctaccgcc cagcgtttgg atggtcgcaa
      121 cgcgaatgcc ttcttccaga gcacgccaca gaatcgatga ctgcagaacg ttcgctttca
      181 ccagagcaaa cagttcgtcg acttcggcat cactcaggcg ccaatcatta aacaggtgag
      241 caccaaagcc cgtacgttca cccgggttca gcttgccgat aatcgccagc acgatgtcgc
      301 gtgccagatc ggcaatcggg gtgcgaaacg cttcagcaac acgaccttcg cccacttgga
      361 taccatcgac cgagcggaac acgaccggac cacgcagtgc aaatgaaacc tggcgaataa
      421 ccacgacatc acctgcaacc actttcaggg cttccgtgat ggtttcccag ccgaactgaa
      481 tcagcttcac ttcggcgaca aaggaacttt ccagcacacc tgcacgcagc agtgcacccg
      541 gagattccgg gaattcaaag ctgtacagtt tttcttccgg aatggtcacc gccagcagag
      601 cttcctggaa aaagaccgcg atgcgaatcg attcttctga gaaagccggc gcagctgcac
      661 gaaccagata attgtgtttg ttcagttgcg aaaacagcag gtcaactgaa cgctggttat
      721 ccagcagcac gatgcgccag gaatgttcat cacgaatact tgccaggaag gcgtccaggc
      781 gcagaacatc caccagacgc acttgttcca gcgcttcaga caggcgagcg tcaccgacga
      841 tcggaatatc cgccgtaa
//
