LOCUS       sequence-76111-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat2
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gaggcgttgc
       61 tgggtgtatt cgaccagcca ctggctcact ttgacccacg gacccgttgc gttaaccaga
      121 ccacgccatt cgtccagcag cagaccacgc agcagatcct gccaattcag ttcgccggcc
      181 acaccccagc cgttagaggt acgcgtcagg ccgaagatcg cacccttggt gcctgaaaag
      241 tacggatcga taaccagacc cgtattggac ttaatttccg gatagatgcc aatgttttta
      301 atttcgtcca gggtaaaatc gatcgcgtaa tagcgcagaa ttgaaccgga gcccagcagc
      361 tgcacacgac cactggtacc gccgatcaga aaaccgcggc caatagcaac cagcactgca
      421 tcgtaacgga aagctgcaga ataacctgcc ggaccagcac ccagcacgac aacttgggta
      481 cgacccagtt ccacttcttc aatttcgcca aacttggctg ccggtgccgg agctgcggct
      541 tcctgacgtt catgatcgac ttcgaaaccc agaacgccca gtttgatacc ttgaataacg
      601 ctttcgtcga tcacgactga ggaggtatag cggccctgtt catcaaccgg tgcattcaga
      661 cgcacaatgg tcgtggtttg gtccggggca tcaccgccgg tacgattcac ttccgtctgc
      721 ttttccagcg catccggcgg ggttttgatg ttgtgcggaa taccaaccac cgcagcttct
      781 gcgatgcgaa cgccttcgtc actcgtaatg accagtttaa cagcatcaga gacttgccat
      841 gcgcgcagcc aaccctgaac ttcatggcct tgacggatct gaatgtcatc ggccttttca
      901 ttcagttcct gacccgcgat ggtgataatt tcgtccagat cacgaatatc acccgtttct
      961 gcc
//
