LOCUS       sequence-76113-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat4
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc gcacgatgac
       61 aatcgtcaga cccgccgggc caatattttt gtagttggac ggaacgttca gcaggtcacg
      121 cagacttgcg gtgaaatcca gttcaccgcg gccatacagc agccaaccgg aacgcagcag
      181 cagcagcagt tcgccgatga ccagttcagc aacacttttc agttgttcgt catccagtgc
      241 accacgaatg cccgacgtca caaacggtga acgcggacct gccagggtac gaacttcgta
      301 cacgttcgcc ttctgcagcg cgaaagcaaa ccaagagcgg atagcattaa tcagacctgc
      361 aacgccttta tcggcgatgt aggtcaggtt gtcacgaata tctgaaaaca cgtcatattg
      421 ctgatcgatc ttcggctgac cgccaacgga caccgtgaac agactgcgat tagccggaaa
      481 cagaccaacc acaccacggt tggtcggacc caggacacct gcaacgtatt tttccagcag
      541 tgccaggact tgggcgcgtt caaccgtaaa accaatggcc tgcagacgac caaccggcgt
      601 ggtcagcgtg ccggtcactg cagccggggt cagttcacgc agggaacttg cggcatcgat
      661 ccaataacct tgccagcccg attcgcgcag cagttcatta ataccgacac gtgattcggc
      721 gccttcgata acctgattgt cgttaatacg cagcgtatcg gtcgcaacca gacccagttc
      781 cttcaccggg atcggattcg gttcaacgaa cagtttccat gcaatatcca ggcgaccgtt
      841 tgcggtcgac acacgagcca ggataccgaa gacttcggcc agaaagcctt tattttctga
      901 accgataaag ccctgggtaa tcaccagacc ttcgttcagg cgagagtgcg gcgccaggaa
      961 gctgtcatca aagcc
//
