LOCUS       sequence-76114-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat5
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agcttcgcca
       61 gttccaggcc aaccactttg taccaataat ctgcaatacc ggcttcaacc gccactttat
      121 tcggggaacc aaagccaatc acggtgcgag cgatgtttgc cagctgttct tccgtacgtg
      181 acagaatcag ggcggtcggg ccatcttgct tattcaggac ctggatgtca acaccttcat
      241 ccggttgcag acgcaggtag aacggaacac ctgcccattt ataccagtcc aggatacgaa
      301 taccctgcca cgtagatgcc agacgtgcat ccacttcggt cgagacacga attgattcga
      361 tgtcgccgct atccgcgaca accgtggtaa actgtttcac accgtccggg gtgccaatca
      421 gagccacatc tgcacccttt tcggcgaccg acgcgataat tttggtcaga atcggtgcaa
      481 ccagttcata ggcttcgcgt gattcgatat tcagtgccag gtttttcaga tccggcaggc
      541 cttcaatttc caggatacga ccgataatca gcggaatctt gcccaggttg gcgtccagca
      601 ggaatttatc accgatattc agaaaaacgc gcgggtcgtt ttctgcttcc agccaacgcg
      661 tcactgcacc ggtgaacagc agaatatcgg cgtgacgttc accccacact gcagccggga
      721 ccttaaccgt cacggtcagc tggtcgcgcg gggtgatatc cagtttcaga accggggcaa
      781 acgcttgacg cagatccagc gtgccgtatt taccgccctg ggtcgccacg acaatcagcg
      841 gagcgtccag cttcgtatcc agcagaattg cggcggtttt accttgctgg atcgcagctg
      901 ccggatc
//
