LOCUS       sequence-76117-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat8
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agcttcagaa
       61 tcgcatcaac gctagctttc agcagttctg ccgtttcttc ggcggtgatt tcacggttga
      121 taccaatcac tttcagctgc agttcatgcg cgatttgctg ttccacaatg acttcgtaac
      181 cacggcccgg cagatcggta taaccgatca ctttgcctgc ggccgtaatc tgaccggtga
      241 aaaagcgttc gaaccacgcc gatgagccag cgatatcggt aatgacacgg taccaattgg
      301 cttccagcag ttcgcgatag aattcagcca gctgaacacc acgcagccag tcatccagac
      361 tcgtgcgggt accaatgcca aaggacagtt gaaccttact ctgcggatcg atgcccaggc
      421 ggataatggt tgcggaaatg ttaaaccacg gcgtttcggc caggtagatt ttgtcggtca
      481 cattaccact gacttccagc agtgcacgca cagagccgct ggcgataatg tgattttcgc
      541 ggtacagcgg gttgatgtcg gtaccgccat caccatactt aatgaacggg tacgtctgca
      601 gatacgattt atccggcgcc cagataatac gcacgccagc gaccagcagg gttgatgccg
      661 gatggcgcag ggcttccgtc agcagaaaat tcttgtcaga aacaatcaga tccaccgcat
      721 tgcttttacc gttgccaaca tacacaccga cgtggtcggc cgtgccctta tgtgcatcgg
      781 ccagggtacc agacgcagca tttttgttcg caaaagccgt gatatcggtt gcttgctgcg
      841 gggtcaggtc cgggacacgg aacagaacca gatcaccggt gcgcggcagt tccaggccaa
      901 attgttcttt atc
//
