LOCUS       sequence-76118-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat9
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agcttttcat
       61 tcactgtcag ggcttgaccg gtcacacggc tcagctccag gataccaggt aaacggcctg
      121 cgccgccggt tgctaacacg gttgcgcgaa cgatgccgcc gaagatgttc actaacacgg
      181 cgcgaaacag ggcgttgcca tctgcgccca gcttaaccac ccaaggacct gcgccgatct
      241 tcagggcctc gccaatatct gctaagctct tcacgcctgc tgcctccagg gctgcgaact
      301 tcacggcttc gtatgtcagg gtgccactac ggccactggc tgcccaaggg aaggtggctg
      361 tctcgtaact gataccgcgg ccgatggcaa ccagcactgc atcatacttt gccacgtgca
      421 gcagggcacg tgtcgggtct tctaacagct cgcgcagcac cggggtcact aaaccacggg
      481 cggctaatgc cggttgtgct gcaggggctg ctgctgcagg tgcgctttca cgaactgtgc
      541 cgcccacctc gtaacccttg cccagaacgt tcaccagcac gttcagctta acattggtgt
      601 ccgggtcgct aatggtgctg ctgtagcgct cgaagtcgta ggtcacggtc ttcagccaag
      661 ggccgccgtc gatcagctca ccaccgaact cttctttcac cgggctaggg gtgccctggt
      721 aataacggat gccgccgcca acaggggttg ttaacgggcc acgttcgaag ctctcggcga
      781 tcaccacgcg aatgttggcg aaggtgccgc gcacgttttc ctc
//
