LOCUS       sequence-76119-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat10
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg aggcgcagac
       61 tattgccgtg ggcggcgatg atcacacggt caatggtcag tgccagactc tcggtcagcg
      121 gcagctcttt atttaagccc tgtaaggcac cgtagtgctt cgggctattg atcaggatac
      181 gaccgatgat gctggccacc ttgtcaacgg cgccggtcag aacagggttg gcgaagaatg
      241 cgcggatttc gttctgcagc tgaggttcct gatcatcgaa ctccagcagg gcgcgattga
      301 tgatgaactc cagacgcacg gcgaagctgg cgttttcatc gtctgcactc agctgggcgt
      361 atgcttcacg gtacagggcg gtcagattca gggtacgacc atcatccagc agcaggatgt
      421 caccaggaac cacgtctgcc ggtaagccct tgcctaagtt ggcgtccagc aggaagcgat
      481 agaagtcgtc ggtgctggtg atttccttca ccagttgagg caccacgccc ttggtgtcta
      541 acaggattgc ggctgtcttc ggattaccca gctggccttg caggtatgcc tcgtttgcct
      601 tcaccacgcc gtagctcacg ctgtcacgca gattgtcggc cagcggtaag ttcagatcga
      661 tggtgccctt ggcacccggg ccaacgatca gacgtgtgcc ttcaatcggg tccactgcga
      721 tatccactgc gtcgccctta ctaatgtggg caatcaggtc cagcaccttc acgaagttgt
      781 cattcagggc aataccggca cgcacggtta agtccacaac gctaacgttc ggtgtcggca
      841 cttttgctgc acctgtgctg ctcgggatga tgttttggct ggcgcccttg atgtagttgc
      901 cctcgttgat gctgtatgtt ttttcgttgg cgaacaggtc cagcttctca acgatgaact
      961 cgcccagggt tttcacgatc acggcgaa
//
