LOCUS       sequence-76120-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat11
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc gctgtgccag gccttcaacg
       61 atggcggtct taatcaccgc gacaccgcca gccagtttac ccaccgtttc atccgaattc
      121 gctgagatgg tgcccacttg tgcaatggcc ttgacaccgt agccgtcgtt gaagataaca
      181 atatcaccca ctttcgcagc tgcggagccc gtcagcacga taccgccggc cgaacgttgt
      241 tcgaacgggg tctgtgacag ccaaaacaga cgttgaaccg gcaccagttc ttccggaatc
      301 agtttatcac cctgggtgta atagcgcgcc aggatgtgaa tgaaagccgg ataaaaacga
      361 ttcgtcgcgg tcacgtccag atctttttca aagttccaca ggatgtcatc cgggtacagc
      421 ggtgcacgca gcagcggacc tgcgacttta ccgctattca ggtccagttc aatatagcca
      481 accttttcca gcaggataat atcggccgct tctttagctg ccaccagttc cgagtcaccg
      541 gtcaggattt tgtactgttc accatccggg ccaatcagtg aacgtgccag cagcagaccg
      601 ttacggatca gcggttcaat cagacggact gcctgagaaa cggccgtgac aacggtgcgc
      661 acttggctga acggacctgc aatagcctgc agtgcacgca gggtaacttc cagcacttta
      721 gagtccaggt agacatcctg aaccgcattg atcaggcgca ccagatcaat cgggttttcg
      781 gacgtcaggc gttcatagat actttcttga accagcgcac gaaaatggtc aatagccagc
      841 ggcacatcgg ccgcactggt ttcgcgaccc agcaccaggt tgaagacacc acgcggcagg
      901 ccaatttcgt caacgatgcg aatca
//
