LOCUS       sequence-76124-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat12
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agacgaatcg
       61 tattgccgac ggtttcgaat tcgtccagtt tcagaacatc gttcgcagca ccaaacgcat
      121 ccagcaggct aatgccacgc gcgaacagcg cttcgataaa atcgtcggca cggaacagag
      181 aaataaagcc gctacccaga ttttctttcg cgatgtcatg aacatcggcc acgtcgcgcg
      241 ccaccgatga gtcaaagacg gtgccatcaa tcagacgagc cgggattgcg ccttcacctt
      301 ggttaataac tttaccgtac gccagttccg gcgggatcac cagacgaccc agcgaagcac
      361 ccagagcata tgctgacttc tgatctgccg ggtcaccgga actacgcagc agcacgccga
      421 cattttcacc ggctttcgcc agaaccgtgg taattgcggc ggtcagcgtg gtcttttcga
      481 tgccttcacc gacttcgaat ttcgcagcaa ccagcacacc aatgcggaaa aaggtcagat
      541 tgccaacaaa cggatcggta gcaatcttga tttctgcttc ggtcagttct tcaccgccca
      601 gataacgaga tgcggccgtc agaatatccg cggtatcgtc atctttgctg ttgatcactt
      661 gcgtggtacc cagtgcaaca ccgcccagct gcaccgcacg cggtgccgga ttgataccat
      721 ccaggttgaa ctggcccaga ctgcgattga ccagatcttc aaccagggat tccagacgca
      781 gttccagact aaattcttct tcctgcgcag cgcggaaatc ccacacgtca aagttttcag
      841 caatacggat gacaatctgt gccggggtct tgtcacccag cagcacgctt tccagagcaa
      901 tgcgtgcatc ttccagaaac gtttcggcga tgtatttcag cagagaggcc agca
//
