LOCUS       sequence-76125-e        9872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of EGFP-tagged human FGD5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Ando K, Fukuhara S, Moriya T, Obara Y, Nakahata N, Mochizuki N
  TITLE     Rap1 potentiates endothelial cell junctions by spatially controlling
            myosin II activity and actin organization.
  JOURNAL   J Cell Biol. 2013 Sep 16;202(6):901-16. doi: 10.1083/jcb.201301115. 
            Epub 2013 Sep 9.
  PUBMED    24019534
REFERENCE   2  (bases 1 to 9872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201301115"; journalName: "J Cell Biol"; date: 
            "2013-09-16- 16"; volume: "202"; issue: "6"; pages: "901-16"
COMMENT     Sequence Label: pEGFP-C1-hFGD5
FEATURES             Location/Qualifiers
     source          1..9872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6660..6781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6751..6770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6788..7243)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6925..6946)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7137..7156
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7270..7374
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7376..7733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7376..7567
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7399..7419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7584..7719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7768..8562
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7768..8562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7822..7841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8432..8451
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8750..8769)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8794..8841
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9170..9758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9659..9678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctaccatg ttcaggggtc cgaagccccc cattgccccc
     1381 aagcccaggc tgactgcccc aaacgagtgg agagccagtg tgtacctgaa tgacagcttg
     1441 aacaaatgca gcaacgggcg gctgccctgt gtagacaggg ggcttgatga ggggccccgg
     1501 tccatcccaa agtgctctga gtcggagacc gacgaggatt acatcgtggt ccccagggtt
     1561 ccgctgaggg aggatgaacc caaggacgag ggcagtgtgg ggaacaaagc cctggtgtct
     1621 cccgagtcct ctgcggaaga ggaagaggag cgtgaagagg gaggcgaggc atgtggcctg
     1681 gagggtacag gagctggtga ggattcagtg gcccctgctg ctccgggtgc aggagcgctg
     1741 agcagggagg gtgaggaagg cacagacctt gctcttgagg atgaagggga gggctgcgct
     1801 gatgagccag ggacactgga gcaggtgtcc agaagtgagg aggaagagaa gctagtgcag
     1861 ccacacaggg agtgcagcct ggaggacagt gggccttggg ctggagaggg ggtcttccag
     1921 agcgacctcc tcctgcctca catccatgga gaggaccagg agccccccga cacccccggg
     1981 gaggcagagg aggatgatga ggaaggctgt gccagcacag acccagcagg ggcagatgag
     2041 ggttcgggtc ctgacaggcc cacggaggac atgggacagg atgctgagga caccagtgag
     2101 gagccccctg agaaggagga gctggccggg gtccaggagg cagagacagc cacagactgc
     2161 cctgaagttc ttgaggaggg atgtgaagag gccacgggtg tcacaggtgg ggaacaggtt
     2221 gacctcagtg aaccacctga ccacgagaag aaaaccaacc aagaagtggc agccgccacc
     2281 ctggaggacc atgcacagga tgagtccgcc gaggagagct gccagattgt cccttttgag
     2341 aatgactgca tggaggactt cgtgacttcc ctcacaggaa gcccctatga gttcttccca
     2401 actgagagca cctctttttg cagcgagagc tgttctcctc tttctgaatc agcgaaaggt
     2461 ttagaatcag agcaggcacc aaagctgggg ctgcgtgcgg aggagaaccc catggtgggg
     2521 gctttgtgtg gccagtgtgg ctccctacag ggtggagcgg ccgagggtcc cgcagcccct
     2581 gatgtggtgg tcgtgctgga ggaggaggcc ttggatgatg cactggccaa cccctatgtg
     2641 atgggagtgg gcctgcccgg tcaggcggcc cctggagaag gagggcaggc tgcatcggac
     2701 gccctgggtg gttatggctc gaaagaagaa ttgaactgtg aggcagaggg tggcctggtt
     2761 cccgcggaca ggaagaacac cagcacgagg gtccggcccc actctgggaa ggtggccggc
     2821 tatgtcccag aaaccgtccc tgaagaaacc ggacctgagg cgggctcgtc agcccctggc
     2881 attggaggtg ccgcagagga ggtgggaaag acgcttttgt cattggaggg gaagcccttg
     2941 gaagccagca gggccttgcc agcaaagccc agggccttta ctttataccc tcggtcgttc
     3001 tccgtggaag gccgagagat tccagtgtcc gtgtaccagg agcctgaggg gtcagggttg
     3061 gatgaccaca ggataaagag gaaagaggac aatctctctc tgtcgtgtgt aattggctcc
     3121 tctgggagtt tctcccagag aaaccacctt ccgtccagcg gcacctccac gccttcttcc
     3181 atggtcgaca tcccacctcc tttcgacctg gcctgcatca ccaagaagcc catcacaaag
     3241 agctctccct cactcctgat cgagagcgac tccccggaca agtacaagaa gaagaagtca
     3301 tcctttaagc gcttcctggc actgacgttt aagaagaaga cggagaacaa attgcatgtg
     3361 gatgtgaacg tgtcttcctc taggtcctct tcagagtcca gctaccacgg gccttccagg
     3421 attctggaag ttgaccggag aagcctcagc aactcccctc agcttaagtc tcggactggg
     3481 aagctccggg cttctgaatc cccctcctcc ctcatctttt atagagatgg caagaggaaa
     3541 ggtgtcccct tcagcaggac ggtgtccaga gtggagtcct ttgaagaccg ctcccggccg
     3601 cccttcctgc ccttgccact gaccaagcca cggtccatct ccttccccag cgctgacact
     3661 tcagactatg agaacattcc agccatgaac tcggactatg agaatatcca gattccaccc
     3721 cggagacctg ccagggctgg cgcgttcacg aagctgtttg aagatcagag cagagccctg
     3781 tccacagcaa acgaaaatga tggctacgtg gacatgagca gcttcaacgc ctttgagagc
     3841 aaacagcaga gtgcagacca ggacgcagaa agcgcctaca cagagcccta caaagtctgt
     3901 cccatctcgt cggcagcccc caaagaggac cttacgtcgg atgaagagca gagaagctcg
     3961 gaggaggagg acagtgcttc aagagacccc agtgtcaccc acaaggtgga aggacagtcc
     4021 agagcccttg tcatcgcaca ggaactgcta tcttcagaga aagcatacgt ggagatgctc
     4081 cagcacttaa atctggattt ccatggagct gtcatgaggg ccttggatga catggaccat
     4141 gaaggcagag acacattggc ccgggaggag ctgaggcagg gcctgagtga actcccagcc
     4201 atccacgacc ttcatcaagg catcctggag gagctggagg aaaggctgtc aaattgggag
     4261 agccagcaga aggtagctga cgtcttcctt gcccgggagc aggggtttga tcaccacgcc
     4321 actcacatcc tgcagttcga caggtaccta ggtctgctca gtgagaattg cctccactct
     4381 ccccggctgg cagctgctgt ccgtgaattt gagcagagtg tacaaggagg cagccagact
     4441 gcgaagcatc ggctgctgcg ggtggttcaa cgcctcttcc agtaccaagt gctcctcaca
     4501 gactatttaa acaacctttg tccggactcc gccgagtacg acaacacaca gggtgcactg
     4561 agcctcatct ccaaagtcac agaccgtgcc aacgacagca tggagcaagg ggaaaacctg
     4621 cagaagctgg tccacattga gcacagcgtc cggggccaag gggatctcct ccagccagga
     4681 agggagtttc tgaaggaagg gacgctgatg aaagtaacag ggaaaaacag acggccccgg
     4741 cacctatttc tgatgaacga tgtgctcctg tacacctatc cccagaagga tgggaagtac
     4801 cggctgaaga acacattggc tgtggccaac atgaaggtca gccgccctgt gatggagaaa
     4861 gtgccctacg ctctaaagat tgagacttcc gagtcctgcc tgatgctgtc tgcgagctcc
     4921 tgtgcagaga gggacgagtg gtatggctgt ctgagcagag ccctccctga ggactacaag
     4981 gcccaggcgc tggctgcatt ccaccatagc gtggagatac gagagaggct gggggttagc
     5041 cttggggaga ggccccccac cctggtgcct gtcacacacg tcatgatgtg catgaactgc
     5101 ggctgcgact tctccctcac cctgcggcgt catcactgtc acgcctgtgg caagatcgtg
     5161 tgccggaact gttcgcggaa caagtacccg ctgaagtacc tgaaggacag gatggccaag
     5221 gtctgcgacg gctgcttcgg ggagctgaag aagcggggca gggctgtccc gggcctgatg
     5281 agagagcggc ctgtgagcat gagcttcccg ctgtcttcac cccgcttctc gggcagtgcc
     5341 ttttcatccg tcttccagag cattaacccc tcgaccttca agaagcagaa gaaagtccct
     5401 tcagccctga cagaggtggc tgcctctgga gagggctctg ccatcagtgg ctatctcagc
     5461 cggtgtaaga ggggcaagcg gcactggaag aagctctggt ttgtcatcaa aggcaaagtt
     5521 ctctacacct acatggccag tgaggacaaa gtggccttgg agagtatgcc tctgctaggc
     5581 ttcaccattg ctccagaaaa ggaagagggc agcagtgaag taggacctat ttttcacctt
     5641 taccacaaga aaaccctatt ttatagcttc aaagcagaag ataccaattc agctcagagg
     5701 tggatcgagg ccatggaaga tgcgagtgtg ttatagcagt tatcaagcat gtggacttgt
     5761 aacaaattct taggtcaata tgtgaatgct tttagaagct aagctgtggc tcaactcatc
     5821 cggacacaca cctggattca gcaatgaggc ctgacctttt ttgctataac cgccccacca
     5881 ctcccctgcc cttgccaaca tcttcatgaa tggaatcctt aagggatatt tatggacctc
     5941 tccttttctg tgttttccac ccctaccccc acccgccacc cagtaataaa ctatttcctt
     6001 accccgcagt gagttaaaat ttagtaagat gaagatgtgg aaaccaatga aaagtacatt
     6061 tagaaatgca ggctttttag tagaaagaag ctcttacagt ttttactcat gataaatctt
     6121 atcacctttc aaaaattgat tgtggtgcct caggtgaaat gagaagctat cagtaacagc
     6181 tactctcatc gaaagcactt tattatttta cggagctgca acctccaatt ttgtgttttc
     6241 taagtgattt acttaagtca ataacagatg gaatcctgtg gactaatgtg ccaccagttt
     6301 tctaacaagg gtgtacataa aatggatttg tgcagacctg gctgtggctt gctggtgtgc
     6361 aagcacacat cactttagag gggctgcgcc ccaccagata ggaaggggag gattttctcc
     6421 tccagtcgac cttgtgacct gtatactaac ataacttatt aaaggagggg tccctcctcc
     6481 cccagagcag tcctcaacac tatgtcagtg ctggaaatgc agccactggc agatccaccg
     6541 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     6601 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6781 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6841 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6901 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6961 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7021 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7081 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7141 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7201 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     7261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     7381 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     7741 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7921 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     7981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     8041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     8101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     8161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     8221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     8281 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     8341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     8401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     8461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     8521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     8581 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     8641 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8701 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     8761 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     8821 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     8881 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     8941 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     9001 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9061 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9121 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9181 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9241 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9301 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9361 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9421 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9481 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9541 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9601 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9661 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9721 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9781 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9841 attctgtgga taaccgtatt accgccatgc at
//
