LOCUS       sequence-76130-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEYellow-Telomere for In Vitro Translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pT7CFE1-TelR15-YPet
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    76..537
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(132..149)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gaattgtaat acgactcact atagggcgaa ttaattccgg ttattttcca ccatattgcc
       61 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
      121 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
      181 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
      241 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
      301 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
      361 gctcacctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
      421 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
      481 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
      541 ggccaccacc catatgggat ccggtatcca cggagtccca gcagccgtag atttgagaac
      601 tttgggatat tcacagcagc agcaggaaaa gatcaagccc aaagtgaggt cgacagtcgc
      661 gcagcatcac gaagcgctgg tgggtcatgg gtttacacat gcccacatcg tagccttgtc
      721 gcagcaccct gcagcccttg gcacggtcgc cgtcaagtac caggacatga ttgcggcgtt
      781 gccggaagcc acacatgagg cgatcgtcgg tgtggggaaa cagtggagcg gagcccgagc
      841 gcttgaggcc ctgttgacgg tcgcgggaga gctgagaggg cctccccttc agctggacac
      901 gggccagttg ctgaagatcg cgaagcgggg aggagtcacg gcggtcgagg cggtgcacgc
      961 gtggcgcaat gcgctcacgg gag
//
