LOCUS       sequence-76133-e         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of untagged gene sequences together 
            with 4 copies of the core DSR motif into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-4XDSR
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(204..221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(204..220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(213..235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cttcgtatag catacattat acgaagttat tgctagccgt tttcttcaat tactaaatta
       61 gaactaattc aatcatatgc tggcatgcgg agagctcgga gtcgacgaga ctagtttaaa
      121 ctccgtatta aacccattct taaacagaac tttaaacccc gggataactt cgtatatggt
      181 attatatacg aagttatgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      361 cgcctgatgc ggtattttct ccttacgcat ctgtgcggna tttcacaccg cataccatcg
      421 tccggatcaa tgaagtagat gagtttacta cctgtatata cttttttttt tgtc
//
