LOCUS       sequence-76135-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALERed-Telomere for In Vitro Translation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pT7CFE1-TelR15-mCherry
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..25
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    75..536
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(131..148)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aattgtaata cgactcacta tagggcgaat taattccggt tattttccac catattgccg
       61 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
      121 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
      181 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
      241 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
      301 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
      361 ctcacctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
      421 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
      481 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
      541 gccaccaccc atatgggatc cggtatccac ggagtcccag cagccgtaga tttgagaact
      601 ttgggatatt cacagcagca gcaggaaaag atcaagccca aagtgaggtc gacagtcgcg
      661 cagcatcacg aagcgctggt gggtcatggg tttacacatg cccacatcgt agccttgtcg
      721 cagcaccctg cagcccttgg cacggtcgcc gtcaagtacc aggacatgat tgcggcgttg
      781 ccggaagcca cacatgaggc gatcgtcggt gtggggaaac agtggagcgg agcccgagcg
      841 cttgaggccc tgttgacggt cgcgggagag ctgagagggc ctccccttca gctggacacg
      901 ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg cggtcgaggc ggtgcacgcg
      961 tggcgcaatg cgctcacggg agc
//
