LOCUS       sequence-7614-ex        4420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Seeler JS, Marchio A, Losson R, Desterro JM, Hay RT, Chambon P, 
            Dejean A
  TITLE     Common properties of nuclear body protein SP100 and TIF1alpha 
            chromatin factor: role of SUMO modification.
  JOURNAL   Mol Cell Biol. 2001 May . 21(10):3314-24.
  PUBMED    11313457
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 May . 21(10):3314-24."
COMMENT     Sequence Label: 6598-HisSUMO1GG
FEATURES             Location/Qualifiers
     source          1..4420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9..200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     279..298
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1052..1063
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1082..1099
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1416..1537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1453..1472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1507..1526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1571..1588)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1742..2330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1822..1841)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2501..3292,3293..3361))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3124..3143
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3362..3466)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3534..3552
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3591..3613)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3713..3732
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3799..4254)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3936..3957)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4148..4167
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4384..4406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4398..4415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4399..4415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct gtgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcca
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattcat taaagaggag aaattaacta tgagaggatc
     1081 gcatcaccat caccatcacg gatctgacca ggaggcaaaa ccttcaactg aggacttggg
     1141 ggataagaag gaaggtgaat atattaaact caaagtcatt ggacaggata gcagtgagat
     1201 tcacttcaaa gtgaaaatga caacacatct caagaaactc aaagaatcat actgtcaaag
     1261 acagggtgtt ccaatgaatt cactcaggtt tctctttgag ggtcagagaa ttgctgataa
     1321 tcatactcca aaagaactgg gaatggagga agaagatgtg attgaagttt atcaggaaca
     1381 aacggggggt taggatccag atcttattaa agcagaactt gtttattgca gcttataatg
     1441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg actctagact
     1561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2161 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3481 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3541 gtatcacgag gcccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3601 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     3661 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     3721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     3781 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3841 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3901 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3961 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4021 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4081 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4141 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4201 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc
     4261 attcgccatt caggctacgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4321 tacgccagct ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     4381 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
//
