LOCUS       sequence-76142-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal 3XHA tagged gene 
            sequences into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-c3HA
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(5..38)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             172..261
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     primer_bind     complement(317..334)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(317..333)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(326..348)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcttataact tcgtatagca tacattatac gaagttattg ctagccgttt tcttcaatta
       61 ctaaattaga actaattcaa tcatatgctg gcatgcggag agctcggagt cgacgagact
      121 agtactggtt ctacaggatc aaccggctcc acggggtcga tgggtagatc ttacccatac
      181 gatgttcctg actatgcggg ctatccgtat gacgtcccgg actatgcagg atcctatcca
      241 tacgacgttc cagattacgc ttaaagatct cccgggataa cttcgtatat ggtattatat
      301 acgaagttat gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
      481 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacca tcgtccggat
      541 caatgaagta gatgagttta ctacctgtat atactttttt ttttgtcaaa gtcactttta
      601 tggcatttcc atgttgaagg attagccaca gcactaaatt ttcagcgtat gattgctttt
      661 aaatatttaa ttttcatcgt tttttaatta atattcaaaa cgatttaatg ccttctacat
      721 ctgaagtcaa gctcatagac tcgggttggg tagctaaaac tacatctaac acgaaggggt
      781 atagccttct taaagtatac tcatgtataa acgttttggg ttcata
//
