LOCUS       sequence-76146-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal yEGFP tagged gene 
            sequences together with the S. pombe spo5 gene DSR element into the 
            S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cyEGFP-spo5DSR
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(6..39)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcttataac ttcgtatagc atacattata cgaagttatt gctagccgtt ttcttcaatt
       61 actaaattag aactaattca atcatatgct ggcatgcgga gagctcggag tcgacgagac
      121 tagtactggt tctacaggat caaccggctc cacggggtcg atgggtagat ctatgtctaa
      181 aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa
      241 tggtcacaaa ttttctgtct ccggtgaagg tgangnagat gctacttacg gtaaattgac
      301 cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac
      361 tttaacttat ggtgttcaat gtttttctag atacccagat cacatgaaac aacatgactt
      421 tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga
      481 cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat
      541 cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata
      601 caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt
      661 taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg accattatca
      721 acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccac
      781 tcaatctgcc ttatccaaag at
//
