LOCUS       sequence-76147-e         371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal 3XHA tagged gene 
            sequences together with 3 copies of the core DSR motif into the S. 
            pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-c3HA-3XDSR
FEATURES             Location/Qualifiers
     source          1..371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(2..35)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             169..258
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tataacttcg tatagcatac attatacgaa gttattgcta gccgttttct tcnnntacta
       61 aattagaact aattcaatca tatgctggca tgcggagagc tcggagtcga cgagactagt
      121 actggttcta caggatcaac cggctccacg gggtcgatgg gtagatctta cccatacgat
      181 gttcctgact atgcgggcta tccgtatgac gtcccggact atgcaggatc ctatccatac
      241 gacgttccag attacgctta aagatcttta aactccgtat tatacccatt cttaancccc
      301 gggatanntn nnnatatgnt attatatacn aagttatgan ntcnnngnnn gncnnnttnn
      361 nncnncnnga c
//
