LOCUS       sequence-7615-ex        4464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Seeler JS, Marchio A, Losson R, Desterro JM, Hay RT, Chambon P, 
            Dejean A
  TITLE     Common properties of nuclear body protein SP100 and TIF1alpha 
            chromatin factor: role of SUMO modification.
  JOURNAL   Mol Cell Biol. 2001 May . 21(10):3314-24.
  PUBMED    11313457
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 May . 21(10):3314-24."
COMMENT     Sequence Label: 6599-SUMO1GG
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9..200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     279..298
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          395..967
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1460..1581
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1497..1516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1551..1570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1615..1632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1786..2374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1866..1885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2545..3336,3337..3405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3168..3187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3406..3510)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3578..3596
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3635..3657)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3757..3776
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(3843..4298)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3980..4001)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4192..4211
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4428..4450
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4442..4459
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4443..4459
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct gtgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcca
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattcgg atccgaattc ggcacgaggt aaaccccgga
     1081 gcgaggttct gcttacccga ggccgctgct gtgcggagac ccccgggtga agccaccgtc
     1141 atcatgtctg accaggaggc aaaaccttca actgaggact tgggggataa gaaggaaggt
     1201 gaatatatta aactcaaagt cattggacag gatagcagtg agattcactt caaagtgaaa
     1261 atgacaacac atctcaagaa actcaaagaa tcatactgtc aaagacaggg tgttccaatg
     1321 aattcactca ggtttctctt tgagggtcag agaattgctg ataatcatac tccaaaagaa
     1381 ctgggaatgg aggaagaaga tgtgattgaa gtttatcagg aacaaacggg gggttaggat
     1441 ccagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca aataaagcaa
     1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1561 caaactcatc aatgtatctt atcatgtctg gtcgactcta gactcttccg cttcctcgct
     1621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1681 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2221 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3541 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcccct
     3601 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3661 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3721 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     3781 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     3841 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3901 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3961 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4021 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4081 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4141 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4201 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4261 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
     4321 acgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     4381 ggggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     4441 ttgtaaaacg acggccagtg aatt
//
