LOCUS       sequence-76152-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA-tagged HIF-1a Ser668 resuide mutated to Ala.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Warfel NA, Dolloff NG, Dicker DT, Malysz J, El-Deiry WS
  TITLE     CDK1 stabilizes HIF-1alpha via direct phosphorylation of Ser668 to 
            promote tumor growth.
  JOURNAL   Cell Cycle. 2013 Oct 25;12(23).
  PUBMED    24189531
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Cycle.
            2013 Oct 25;12(23)."
COMMENT     Sequence Label: HA-HIF1a (S668A)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttaccatg gcctacccct acgacgtgcc cgactacgcc
      121 tccctcggat ccgccaccat ggagggcgcc ggcggcgcga acgacaagaa aaagataagt
      181 tctgaacgtc gaaaagaaaa gtctcgagat gcagccagat ctcggcgaag taaagaatct
      241 gaagtttttt atgagcttgc tcatcagttg ccacttccac ataatgtgag ttcgcatctt
      301 gataaggcct ctgtgatgag gcttaccatc agctatttgc gtgtgaggaa acttctggat
      361 gctggtgatt tggatattga agatgacatg aaagcacaga tgaattgctt ttatttgaaa
      421 gccttggatg gttttgttat ggttctcaca gatgatggtg acatgattta catttctgat
      481 aatgtgaaca aatacatggg attaactcag tttgaactaa ctggacacag tgtgtttgat
      541 tttactcatc catgtgacca tgaggaaatg agagaaatgc ttacacacag aaatggcctt
      601 gtgaaaaagg gtaaagaaca aaacacacag cgaagctttt ttctcagaat gaagtgtacc
      661 ctaactagcc gaggaagaac tatgaacata aagtctgcaa catggaaggt attgcactgc
      721 acaggccaca ttcacgtata tgataccaac agtaaccaac ctcagtgtgg gtataagaaa
      781 ccacctatga cctgcttggt gctgatttgt gaacccattc ctcacccatc aaatattgaa
      841 attcctttag atagcaagac tttcctcagt cgacacagcc tggatatgaa attttcttat
      901 tgtgatgaaa gaattaccga attgatggga tatgag
//
