LOCUS       sequence-76161-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of cytosolic S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-HA-SrtAstrep (pKS78)
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..474
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    33..150
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     445..468
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             513..539
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             549..566
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gactcactat agggcgaatt ggagctctag tacggattag aagccgccga gcgggtgaca
       61 gccctccgaa ggaagactct cctccgtgcg tcctcgtctt caccggtcgc gttcctgaaa
      121 cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt
      181 atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatgaacgaa
      241 tcaaattaac aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa
      301 ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca aaaactgcat
      361 aaccacttta actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtaa
      421 taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa
      481 ccccggattc tagagccacc atgcgcagca gctacccata cgacgtacca gactacgcaa
      541 gcagcggcct ggtgccgcgc ggatccgtct tgcaagcaca aatggcggct cagcaacttc
      601 ctgttatagg gggcattgcc ataccagagc ttggcattaa tttaccaatt tttaaaggtt
      661 taggaaatac tgagcttatt tatggcgcag gaacgatgaa agaagaacaa gttatgggag
      721 gagaaaataa ttattctctt gccagtcatc atatttttgg aattacaggt tcatctcaaa
      781 tgctcttttc gccgcttg
//
