LOCUS       sequence-76165-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of cytosolic S. pyogenes SrtA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pcDNA3.1(-)-HA-SrtAstrep (pKS77)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             129..155
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             165..182
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(790..807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    796..907
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaacgg gccctctaga gccaccatgc
      121 gcagcagcta cccatacgac gtaccagact acgcaagcag cggcctggtg ccgcgcggat
      181 ccgtcttgca agcacaaatg gcggctcagc aacttcctgt tatagggggc attgccatac
      241 cagagcttgg cattaattta ccaattttta aaggtttagg aaatactgag cttatttatg
      301 gcgcaggaac gatgaaagaa gaacaagtta tgggaggaga aaataattat tctcttgcca
      361 gtcatcatat ttttggaatt acaggttcat ctcaaatgct cttttcgccg cttgaaagag
      421 cacaaaatgg gatgtccatc tatttaacag ataaagaaaa aatttacgaa tacatcataa
      481 aagatgtttt cacggtagct cctgaacgcg ttgatgttat cgatgataca gctggtctca
      541 aagaagtgac tttagtgact tgtacagata tcgaagcaac agaacgtatt attgtcaaag
      601 gagaactaaa aacagaatac gactttgata aagcgcccgc cgatgtattg aaagctttta
      661 atcattctta taaccaagta tctacctagc tcgagcggcc gccactgtgc tggatatctg
      721 cagaattcca ccacactgga ctagtggatc cgagctcggt accaagctta agtttaaacc
      781 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      841 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      901 ttgcatcgca ttgtctgagt
//
