LOCUS       sequence-76166-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chlamy Cp transformation for transgene integration via 
            double-homologous recombination into the rbcL-psaB intergenic 
            region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Chen HC, Melis A
  TITLE     Marker-free genetic engineering of the chloroplast in the green 
            microalga Chlamydomonas reinhardtii.
  JOURNAL   Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. 
            Epub 2013 May 7.
  PUBMED    23647698
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.12073";
            journalName: "Plant Biotechnol J"; date: "2013-09"; volume: "11"; 
            issue: "7"; pages: "818-28"
COMMENT     Sequence Label: pBS-67Sma
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     48..64
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg ggtaccgggc cccccctcga ggtcgacggt
       61 atcgataagc ttgatatcga attcaaaatt gcgcagaatc gaagggtatg tcttcgaagc
      121 atatgcagtt gggtaaggca cttgtcctct tatacttcag aaatataaag tatactcaaa
      181 tgttactcgt ggatggcttt ggtatttagg cgtagcatgc agtaaatata tggccaatgg
      241 ctcctctttt agcaagttct caacttaaaa tgcaatctct taacgagaat ctaaagctca
      301 gaaatatatt tgaattaaaa tttcccacag gattatggcg tagtcataat atcaactaaa
      361 aaatcttttt aaattttaaa atttactttt ttacgctttt gtatgcaaag tttgctttgc
      421 acctgaatag ttttattaaa tttttattta atggtagttt aatagtagta atttacttca
      481 attaaacaaa aaaaatccta attgtttatc cctttaaaag agcgcttaaa gtttttttac
      541 ttagtgaagt aaaaataccg ctcccttctg gtattttttc ttttgattta acaattagca
      601 ttttaacctt ttacttttct ctcagtgtta tactgcttaa aagtttttag gtcattagat
      661 aatatttaat aatattacat atagggagta agacaatttt atggcaacta aactgtttcc
      721 aaaatttagc caaggtctag ctcaagaccc tactacacgt agaatttggt atgggcttgc
      781 tatggctcat gactttgaaa gccatgatgg tatgacagaa gaaaatcttt accaaaagat
      841 ttttgcttcg cacttcggtc aattatctat cattttctta tgg
//
