LOCUS       sequence-76168-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chlamy Cp transformation for transgene integration via 
            double-homologous recombination into the rbcL-psaB intergenic 
            region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Chen HC, Melis A
  TITLE     Marker-free genetic engineering of the chloroplast in the green 
            microalga Chlamydomonas reinhardtii.
  JOURNAL   Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. 
            Epub 2013 May 7.
  PUBMED    23647698
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.12073";
            journalName: "Plant Biotechnol J"; date: "2013-09"; volume: "11"; 
            issue: "7"; pages: "818-28"
COMMENT     Sequence Label: pBS-67Sma
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgactcacta tagggcgaat tggagctcca ccgcggtggc ggctgctcta gaactagtgg
       61 atcccaggaa ttcaagtaat tcacctgaca ttgctttttc taaaccataa atacgagcaa
      121 taccgtcacc tacttggaaa acgataccga aatctaccat tttcacttct ggagtgtatt
      181 gttcaattaa atctttaata agattactaa gttcttctgg agtacgcatt gccataaaaa
      241 agaaaaaata aataaaagat taaaaaagtt tatttttaaa atctttctcg agaattttaa
      301 ataagtttaa aattcaacaa aaatagtgag tggtaagatc acttgttaac aaaagtaatg
      361 gttcaccctt gtcatattta aatactaaaa ttcatttgcc cgaagaggac aaatttattt
      421 attgcattaa aatccctaag tttacttgcc cgtaagggga agggggggac gtccacaggc
      481 gtcgtaagca actaaagttt atgacgccga ttgctttgtt aggaaaatat aaatatccca
      541 taagaaaagg tcctttaaag gttttatgga ctaaataaaa aagatagcat aagcattaaa
      601 atcatgcaaa ttaaaaaaaa ggtaaatgta tttataaaaa ggtaaatgta tttatatagt
      661 atttatatta tagcataata ataaatatat ttataaattg attgttctta gagctaaaag
      721 agaagaacaa tgggtttata ggtattttga
//
