LOCUS       sequence-76169-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of S. pyogenes SrtA at the plasma membrane in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(788..805)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    794..905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgtttaaacg ggccctctag agccaccatg
      121 ggatgtataa aatcaaaagg gaaagacagc gcgggagcaa tcgatgccta cccatacgac
      181 gtaccagact acgcacatag tgctggtgga tccgtcttgc aagcacaaat ggcggctcag
      241 caacttcctg ttataggggg cattgccata ccagagcttg gcattaattt accaattttt
      301 aaaggtttag gaaatactga gcttatttat ggcgcaggaa cgatgaaaga agaacaagtt
      361 atgggaggag aaaataatta ttctcttgcc agtcatcata tttttggaat tacaggttca
      421 tctcaaatgc tcttttcgcc gcttgaaaga gcacaaaatg ggatgtccat ctatttaaca
      481 gataaagaaa aaatttacga atacatcata aaagatgttt tcacggtagc tcctgaacgc
      541 gttgatgtta tcgatgatac agctggtctc aaagaagtga ctttagtgac ttgtacagat
      601 atcgaagcaa cagaacgtat tattgtcaaa ggagaactaa aaacagaata cgactttgat
      661 aaagcgcccg ccgatgtatt gaaagctttt aatcattctt ataaccaagt atctacctag
      721 gaattccacc acactggact agtggatccg agctcggtac caagcttaag tttaaaccgc
      781 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      841 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      901 gcatcgcatt gtctgagta
//
