LOCUS       sequence-76170-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Galactose-inducible expression of ER-luminal S. pyogenes SrtA in 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Strijbis K, Spooner E, Ploegh HL
  TITLE     Protein ligation in living cells using sortase.
  JOURNAL   Traffic. 2012 Jun;13(6):780-9. doi: 
            10.1111/j.1600-0854.2012.01345.x. Epub 2012 Mar 23.
  PUBMED    22348280
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2012.01345.x"; journalName: "Traffic"; date: 
            "2012-06"; volume: "13"; issue: "6"; pages: "780-9"
COMMENT     Sequence Label: pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..478
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    37..154
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     449..472
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 atacgactca ctatagggcg aattggagct ctagtacgga ttagaagccg ccgagcgggt
       61 gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct
      121 gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc
      181 ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa
      241 cgaatcaaat taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg
      301 gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact
      361 gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat
      421 gtaataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa
      481 aaaaccccgg attctagaat gtttaaatct gttgtttatt caattttagc cgcttctttg
      541 gccaatgcag gtaccattcc cttaggatcc agtgtcttgc aagcacaaat ggcggctcag
      601 caacttcctg ttataggggg cattgccata ccagagcttg gcattaattt accaattttt
      661 aaaggtttag gaaatactga gcttatttat ggcgcaggaa cgatgaaaga agaacaagtt
      721 atgggaggag aaaataatta ttctcttgcc agtcatcata tttttggaat tacaggttca
      781 tctcaaatgc tcttttcgcc gcttgaaaga gcacaaaatg ggatgtccat ctatttaaca
      841 gataaagaaa aaatttacga atacatcata aaagatgttt tcacggtagc tcctgaacgc
      901 gttgatgtta tcgatgatac agctggtctc aaagaagtga cttta
//
